1087 The efficacy of new cultivar of paeonia lactiflora flower extract ‘Yumesaika’

The roots of Paeonia lactiflora are traditionally used as one the herbal medicine. YUMESAIKA, which National Institutes Biomedical Innovation, Health and Nutrition (NIBIOHN) cultivates, has some unique features, such heavy dry root, high amount paeoniflorin. However, flower is removed because promoting enlargement roots. Although root available for cosmetic ingredients, there no ingredient on this new cultivar. Therefore, we evaluated efficacy YUMESAIKA ingredients. First, focused DPPH antioxidants activity enhancement hyaluronan synthase (HAS1, HAS2) gene expressions selection parts (root, flower) cultivars (Kitasaisho, No.205 – other candidate cultivar, YUMESAIKA) Paenia lactiflora. In a plant, expression from extract was higher than that extract. Also, in cultivar plants, those highest three. selected ingredient. Next, studied extract, inhibition tyrosinase or elastase, proliferation skin fibroblast by MTT assay, anti-glycation antioxidant activity. We found showed effects cells, Then, also investigated compound Yumesaika ingredients byHP LC, LC-MSMS. kinds compounds, 1,2,3,4,6-penta-O-galloyl-b-D-glucose, kaempferol-3-glucoside. these compounds were penetrated into thorough permeation assay using reconstruction model. would lead improvement its compounds.